SEEDs enable enrichment of cells with transgene integrations To develop a method for cells with transgene integrations to be enriched through negative selection, we designed two reagents: (1) a guide RNA (gRNA) targeting an intron of a surface-expressed protein that generates a D…

Frontiers | Answered and Unanswered Questions in Early-Stage Viral Vector Transduction Biology and Innate Primary Cell Toxicity for Ex-Vivo Gene Editing REVIEW article Front. Immunol. , 28 May 2021 Sec. Vaccines and Molecular Therapeutics Volume 12 - 2021 | https://doi.org/10.338…

T cell isolation and culture Human peripheral blood Leukopaks enriched for peripheral blood mononuclear cells (PBMCs) from deidentified healthy donors were purchased from StemCell Technologies (200-0092). CD3 + T cells were isolated using the EasySep Human T cell isolation kit (1…

About the Program The Registered Nursing Program at Bakersfield College prepares students to become skilled and compassionate Registered Nurses (RNs). Nursing combines knowledge from science and the arts to help patients of all ages and backgrounds. The program focuses on buildin…

Federal Register :: Duke Energy Progress, LLC; H.B. Robinson Steam Electric Plant, Unit No. 2; Final Environmental Impact Statement Skip to Content Sections Money Environment World Science & Technology Business & Industry Health & Public Welfare Browse Agencies Topics (CFR Indexi…

Gebauer, F., Schwarzl, T., Valcárcel, J. & Hentze, M. W. RNA-binding proteins in human genetic disease. Nat. Rev. Genet. 22 , 185–198 (2021). Article CAS PubMed Google Scholar Hentze, M. W., Castello, A., Schwarzl, T. & Preiss, T. A brave new world of RNA-binding proteins. Nat. R…

Introduction Cells manipulate the shape of their plasma membranes to execute physiological functions ranging from building the protrusions that drive cell motility to coordinating the membrane deformations that enable endocytosis. Actin polymerization plays a major role in coordi…

Abstract CRISPR/Cas9-based gene-editing technologies offer promise for treating inherited retinal diseases (IRDs), however safe and efficient ocular delivery of precision editors remains challenging. To address this challenge, we report a class of Coomassie brilliant blue (CBB)-d…

Delivering ribonucleoproteins (RNPs) for in vivo genome editing is safer than using viruses encoding for Cas9 and its respective guide RNA. However, transient RNP activity does not typically lead to optimal editing outcomes. Here we show that the efficiency of delivering RNPs can…

HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation. Nat Commun. 2026 Jan 15; 17(1):1570. Yang Y, Murrali MG, Galvan S, Wang Y, Stephen C, Ajjampore N, Wang X, Feigon J . PMID: 41540012; PMCID: PMC12901311 . View in: PubMed Mentions: Fi…

Journal of Franco-Irish Studies Volume 6 Issue 1 Article 6 2019 Reclaiming Their Rightful Place?: an Analysis of the Links Between Medieval and Early Modern Female Brewers and Consumers in Ireland With Their Modern Counterparts Christina Wade Trinity College Dublin, [email protec…

Abstract We describe a basic, fast, and reliable technique to isolate and characterize ribonucleoprotein (RNP) using antibody to a constituent protein. The antibody serves to immunopurify RNP from total cells or nuclear and cytoplasmic cell fractions under conditions that promote…

Introduction Community engagement is increasingly recognized as an integral element of ethical global health research [ Reference Adhikari, Pell and Cheah 1 ]. Engaging community members in health research occurs on a spectrum, and their involvement as early as possible to set pr…

Padgett et al. BMC Global and Public Health https://doi.org/10.1186/s44263-025-00140-2 (2025) 3:28 CORRESPONDENCE BMC Global and Public Health Open Access Analytic methodology for demographic variation analyses for wave 1 of the global flourishing study R. Noah Padgett1,2, Matt B…

Experimental model and study participant details Patient tissue samples The LuCaP prostate cancer Patient Derived Xenograft (PDX) tissue microarrays (TMAs) have been described previously. 75 The LuCaP PDX TMA for UCHL1 staining includes 21 adeno-CRPC PDXs and 4 NEPC PDXs with thr…